منابع مشابه
Pyridine nucleotide specificity of barley nitrate reductase.
NADPH nitrate reductase activity in higher plants has been attributed to the presence of NAD(P)H bispecific nitrate reductases and to the presence of phosphatases capable of hydrolyzing NADPH to NADH. To determine which of these conditions exist in barley (Hordeum vulgare L. cv. Steptoe), we characterized the NADH and NADPH nitrate reductase activities in crude and affinity-chromatography-purif...
متن کاملPyridine Nucleotide Transhydrogenase
established unequivocally that either a hydrogen or electron transfer was involved in this reaction. The possibility of a transfer of the monoester phosphate group or an exchange of reduced and oxidized nicotinamidecontaining moieties (nucleoside, nucleotide, etc.) was ruled out by this and other experiments. There still remained, however, the problem of distinguishing experimentally between th...
متن کاملPyridine nucleotide synthesis
The synthesis of NAD and NADP by rat adipose tissue was measured in vitro. Nicotinamide-7-14C and NaHPP04 were incorporated together into NAD with a =P/14C ratio of 1.82 and nicotinic-7-14C acid and NaH232POa with a ratio of 1.94. Nicotinic acid stimulated, by 90%, lipogenesis from glucose-UW by rat adipose tissue in vitro. Glucose plus insulin and refeeding for 48 hr after a 48 hr fast markedl...
متن کاملPhotosynthetic pyridine nucleotide reductase. I. Partial purification and properties of the enzyme from spinach.
In 1939, Hill (1) reported the fundamental discovery that isolated chloroplasts possess the ability to affect a portion of the over-all reaction of photosynthesis; namely, the photolysis of water. In the original experiments of Hill, ferric oxalate was used as the hydrogen acceptor. During the past 20 years, a variety of substances has been found to function as oxidant for the Hill reaction (2)...
متن کاملComplete pyridine-nucleotide-specific conversion of an NADH-dependent ferredoxin reductase.
The coenzyme specificity of enzymes is one of the critical parameters for the engineered production of biological compounds using bacteria. Since NADPH is produced abundantly in photosynthetic organisms, conversion of an NADH-specific enzyme into an NADPH-specific one is a useful approach for the efficient carbon-neutral production of biological compounds in photosynthetic organisms. In the pre...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1954
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)71316-1